Devices and methods for separating particles from a liquid
AssigneeStemCell Technologies Inc.
InventorsEric Jervis, Tom Glawdel
Life of this patent
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Abstract
Surfaces contactable by a bulk liquid for use in particle isolation are described. The surfaces comprise a plurality of ribs spaced apart to capillarily retain a portion of the bulk liquid and particles of interest therebetween. Collectively, the portion of the bulk liquid retained by the plurality of ribs on the surface forms a liquid film. The one or more particles received within the space and enveloped by the liquid film may be protected from one or more forces exerted by a draining meniscus passing over the surface.
Claims20
An apparatus for separating particles from a bulk liquid, the apparatus comprising:
- a container having:
- a surface to be contacted by a particle-containing bulk liquid,
- a plurality of ribs on the surface, each rib of the plurality of ribs being positioned between and spaced apart from two adjacent ribs on the surface by a substantially uniform pitch distance;
- and
- a space between each rib and each of the two adjacent ribs being dimensioned to capillarily retain therebetween a portion of the bulk liquid and at least a portion of the particles entrained therein when the liquid contacting the surface is removed away from the surface,
- wherein the surface is an inner sidewall of the container extending from an upper end having an opening to a bottom wall opposed to the opening, and the plurality of ribs extend away from the inner sidewall toward an interior of the container, and
- wherein the container is a tube, and
- wherein the plurality of ribs extend in a direction between the opening and the opposed bottom wall along the inner sidewall and are distributed around an inner perimeter of the sidewall of the tube and the pitch distance is less than about 1 mm.
A container for holding a particle-containing bulk liquid, the container comprising:
- a closed bottom end having a bottom wall, an open upper end, one or more sidewalls extending from the bottom wall to the upper end along a longitudinal axis in a direction from the open upper end to the closed bottom end, and an inner surface bounding an interior of the container and an opposed outer surface;
- a plurality of ribs on the inner surface, the plurality of ribs comprising a pair of end ribs extending parallel to opposed edges of the inner surface and a plurality of intervening ribs flanked by the pair of end ribs, wherein each rib of the plurality of intervening ribs (i) is spaced apart from two adjacent ribs by a substantially uniform pitch distance, (ii) extends in a direction along the longitudinal axis, and (iii) extends away from the inner surface toward an interior of the container;
- and
- a substantially uniform space between adjacent ribs of the plurality of ribs,
- whereby when bulk liquid is contained in the interior of the container the bulk liquid contacts the inner surface, the plurality of ribs, and the spaces there, and whereby the plurality of ribs are dimensioned to capillarily-retain therebetween a portion of the bulk liquid and at least a portion of the particles entrained therein when the bulk liquid contacting the surface is removed away from the surface.
A method for separating particles from a particle-containing bulk liquid in a container having a surface that is an inner sidewall extending from an upper end having an opening to a bottom wall opposed to the opening, a plurality of ribs extending along the inner sidewall and away from the inner sidewall toward an interior of the container, each rib of the plurality of ribs being positioned between and spaced apart from two adjacent ribs on the surface by a uniform pitch distance sufficient to receive a diameter of at least one particle, and a space between the first rib and the second rib, the method comprising:
- contacting the inner sidewall with the particle-containing bulk liquid, whereby the particle-containing bulk liquid contacts the surface, the first and second ribs, and the space between the first and second ribs;
- receiving at least a portion of the particles in the particle-containing bulk liquid into the space between the first and second ribs;
- removing, by pour-off, pipetting or aspiration, the particle-containing bulk liquid away from the surface in a direction along the axis in which each of the plurality of ribs extend, a portion of the particle-containing bulk liquid received in the space being capillarily-retained in the space between the first and second ribs to form a liquid film on the inner sidewall;
- shielding the particles of the portion of the particle-containing bulk liquid retained in the space between the first and second ribs by being entrained in the liquid film from one or more forces of a draining meniscus as residual bulk liquid and any residual particles therein is removed away from the surface;
- and
- resuspending in a buffer the shielded particles entrained within the liquid film,
- wherein the container is a tube comprising one sidewall and the plurality of ribs extend in a direction between the opening and the opposed bottom wall along the sidewall.
Dependencies are inferred from the claim text.