Cryo formulation-based microneedle device for transdermal delivery of bioactive therapeutic agents and cancer immunotherapy using a cryo-microneedle patch

A cryo formulation-based microneedle device for transdermal delivery of bioactive therapeutic agents, comprising:

• one or more microneedle patches each including an array of miniaturized needles, each miniaturized needle defining a base end and a tip;
• and
• a substrate to which the base end of the array of miniaturized needles is attached or integrated thereto;
• wherein the microneedle patches are in a cryo status;
• wherein each of the one or more microneedle patches are adapted to be applied on a skin surface, in which the miniaturized needles penetrate into skin;
• wherein the one or more microneedle patches consist of a matrix solution and a plurality of biological cells as the one or more bioactive therapeutic agent;
• wherein the miniaturized needles are further arranged to melt so as to release one or more bioactive therapeutic agents into the skin to achieve a targeted therapeutic effect;
• and
• wherein the matrix solution consists of water and a cryoprotectant, the cryoprotectant comprises 2.5% wt of dimethyl sulfoxide (DMSO) and 100 to 200 mM sucrose.