Berberine/mineralized collagen-based composite membrane and preparation method and application thereof

A preparation method of a berberine/mineralized collagen-based composite membrane comprising the following steps:

• (1) mixing chitosan and mineralized collagen with a glacial acetic acid solution to obtain a resulting mineralized collagen suspension;
• (2) mixing polycaprolactone, water-soluble compounds, and berberine with a solvent to obtain an electrospinning solution;
• and
• (3) casting the resulting mineralized collagen suspension obtained in step (1) into a film to obtain a mineralized collagen membrane, and electrospinning the electrospinning solution obtained in step (2) and receiving resulting electrospun fibers on the mineralized collagen membrane to obtain the berberine/mineralized collagen-based composite membrane;
• or electrospinning the electrospinning solution obtained in step (2) to obtain a berberine nanofiber membrane, coating the resulting mineralized collagen suspension obtained in step (1) on the berberine nanofiber membrane, and drying the coated berberine nanofiber membrane to obtain the berberine/mineralized collagen-based composite membrane;
• wherein in the berberine/mineralized collagen-based composite membrane, the berberine nanofiber membrane comprises components of the following mass fractions:
• 20-65 wt % of polycaprolactone, 18-80 wt % of water-soluble compounds, and berberine≤0.1 wt % based on the mass of the berberine nanofiber membrane;
• and the mineralized collagen membrane comprises components of the following mass fractions:
• 15-90 wt % of chitosan, and 10-85 wt % of mineralized collagen based on the mass of the mineralized collagen membrane;
• the mixing processes in step (2) are as below:
• berberine is dissolved in the solvent to get a berberine solution, then polycaprolactone and the water-soluble compounds are respectively dissolved in the berberine solution to get a polycaprolactone-berberine solution and a water-soluble compound-berberine solution;
• the polycaprolactone-berberine solution and the water-soluble compound-berberine solution are mixed to get the electrospinning solution;
• and
• wherein the mass concentration of polycaprolactone in the polycaprolactone-berberine solution is 5-15%;
• the mass concentration of the water-soluble compounds in the water-soluble compound-berberine solution is 10-30%;
• the volume ratio of the polycaprolactone-berberine solution to the water-soluble compound-berberine solution is 7:(2-4);
• the concentration of berberine in the electrospinning solution is 5-50 μmol/L;
• the solvent is a mixed solvent of trichloromethane and methanol.