Compositions and methods for treating wounds

A method of treating a skin wound, the method comprising:

• (1) treating the wound surface by applying a sterile therapeutic composition consisting essentially of recombinant human platelet-derived growth factor BB homodimer (rhPDGF-BB) optionally in a physiologic solution and a porous biocompatible carrier to the wound surface, wherein the porous biocompatible carrier is a collagen sponge or collagen wound dressing or a gelatin, and wherein the carrier provides a substrate for cell attachment and vascular ingrowth as the wound heals, and said applying delivers at least about 10 μg rhPDGF-BB per cm2 of treated wound surface area up to 100 μg rhPDGF-BB per cm2 treated wound surface area, optionally wherein prior to treating the wound surface the wound is debrided to remove necrotic or infected tissue;
• (2) optionally covering the wound with a dressing;
• (3) optionally using a treatment regimen of repeating steps (1) - (2) for 2 to 20 times at treatment intervals of three or more days;
• and
• (4) applying a skin-substitute composition to the treated wound and optionally covering the wound with a second dressing.