Compositions and methods for delivery of nucleic acids to cells
AssigneeYale University
InventorsElias Quijano, Peter Glazer
Life of this patent
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Abstract
Compositions and methods of use thereof for delivering nucleic acid cargo into cells are provided. The compositions typically include (a) a 3E10 monoclonal antibody or an antigen binding, cell-penetrating fragment thereof; a monovalent, divalent, or multivalent single chain variable fragment (scFv); or a diabody; or humanized form or variant thereof, and (b) a nucleic acid cargo including, for example, a nucleic acid encoding a polypeptide, a functional nucleic acid, a nucleic acid encoding a functional nucleic acid, or a combination thereof. Elements (a) and (b) are typically non-covalently linked to form a complex.
Claims22
A method for treating a cancer in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a composition comprising a non-covalent complex of (a) an antibody or antigen-binding fragment thereof, and (b) a therapeutic polynucleotide,
- wherein the antibody or antigen-binding fragment thereof comprises VH complementarity determining regions (CDRs) having the amino acid sequences of SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18, and VL CDRs having the amino acid sequences of SEQ ID NO:24, SEQ ID NO:25, and SEQ ID NO:26;
- and wherein the cancer comprises one or more cells that express a functional ENT2 transporter.
Dependencies are inferred from the claim text.