Coronavirus vaccine comprising a mosaic protein

A nucleic acid construct comprising a first nucleic acid sequence operatively linked to a first promoter, a second nucleic acid sequence operatively linked to a second promoter, and a third nucleic acid sequence operatively linked to a third promoter, wherein:

• (i) the first nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 10, wherein the second nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 4, and wherein the third nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 2, SEQ ID NO:
• 6, or SEQ ID NO:
• 14;
• (ii) the first nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 12, wherein the second nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 4, and wherein the third nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 2, SEQ ID NO:
• 6, or SEQ ID NO:
• 14;
• or
• (iii) wherein the first nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 10, wherein the second nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 12, and wherein the third nucleic acid sequence comprises the nucleic acid sequence of SEQ ID NO:
• 2, SEQ ID NO:
• 4, SEQ ID NO:
• 6, or SEQ ID NO:
• 14.

A nucleic acid construct comprising a first nucleic acid sequence encoding a first MoCoV S protein, a second nucleic acid sequence encoding a second MoCoV S protein, and a third nucleic acid sequence encoding a third MoCoV S protein, wherein:

• (i) the first MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 9, wherein the second MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 3, and wherein the third MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 1, SEQ ID NO:
• 5, SEQ ID NO:
• 13, SEQ ID NO:
• 15, SEQ ID NO:
• 16, or SEQ ID NO:
• 17;
• (ii) the first MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 11, wherein the second MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 3, and wherein the third MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 1, SEQ ID NO:
• 5, SEQ ID NO:
• 13, SEQ ID NO:
• 15, SEQ ID NO:
• 16, or SEQ ID NO:
• 17;
• or
• (iii) the first MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 9, wherein the second MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 11, and wherein the third MoCoV S protein comprises the amino acid sequence of SEQ ID NO:
• 1, SEQ ID NO:
• 3, SEQ ID NO:
• 5, SEQ ID NO:
• 13, SEQ ID NO:
• 15, SEQ ID NO:
• 16, or SEQ ID NO:
• 17.