Composition derived from mammalian umbilical cord and Whartons jelly for use in therapeutic and regenerative applications

A method of preparation of a solution used for therapeutic and regenerative applications having a concentration of at least 0.05 micrograms of total protein extract, the method comprising the steps of:

• a) collecting an umbilical cord;
• b) incubating the umbilical cord in a solution containing antibiotics for sterilization at a temperature not exceeding 40 degrees Centigrade;
• c) washing the umbilical cord in a first buffered salt solution;
• d) homogenizing the umbilical cord in a second buffered salt solution so as to produce an aqueous solution comprising umbilical cord tissue proteins, cytokines, growth factors, and RNA, wherein said proteins comprise Brain Derived Neurotropic Factor (BDNF), Glial Derived Neurotrophic Factor (GDNF), Stromal Cell Derived Factor 1 (SDF-1), Angiopoietin-2, Chemokine Ligand 16 (CXCL-16), Neutrophil-Activating Protein-2 (Nap-2), Glucocorticoid-induced Tumor Necrosis Factor Receptor (GITR), and Fibroblast Growth Factor 20 (FGF-20);
• e) applying an ultrasonic processer to the aqueous solution to sonicate and disrupt umbilical cord tissue, wherein the ultrasonic processor is applied at a power output of 300 W at a frequency of 20 kHz, wherein sonication occurs in 3 second cycles of 2 seconds on and 1 second off for a total duration of 5 minutes;
• f) placing the aqueous solution into a centrifuge at about 5,500-7,500 rpm to separate the aqueous solution into a soluble component and a non-soluble component;
• g) filtrating the non-soluble component of the aqueous solution from the centrifuge through a 0.2 micron filter and discarding the non-soluble component that was filtered;
• and
• h) measuring protein concentration of the soluble component to ensure at least 0.05 microgram of total protein extract.