Second near-infrared window / first near-infrared window dual-mode fluorescence tomography system and method

A second near-infrared window/first near-infrared window dual-mode fluorescence tomography method, applied to a second near-infrared window/first near-infrared window dual-mode fluorescence tomography system, wherein the second near-infrared window/first near-infrared window dual-mode fluorescence tomography system comprises:

• a lighting module configured to irradiate a mouse with a white light and collect white light images, wherein the lighting module comprises a white light emitting device configured to emit the white light, and the white light emitting device has an adjustable luminous intensity;
• an excitation module configured to irradiate a tumor region of the mouse with a second near-infrared window excitation light and a first near-infrared window excitation light such that fluorescent dye in the tumor region emits fluorescence, wherein the excitation module comprises a laser configured to emit the second near-infrared window excitation light and the first near-infrared window excitation light with tunable wavelengths;
• a first near-infrared window collection module configured to collect first near-infrared window white light images and first near-infrared window fluorescent images formed by the fluorescence emitted by the fluorescent dye in the tumor region, wherein the first near-infrared window collection module comprises a first near-infrared window camera configured to collect the first near-infrared window fluorescent images and the first near-infrared window white light images;
• a second near-infrared window collection module configured to collect second near-infrared window white light images and second near-infrared window fluorescent images formed by the fluorescence emitted by the fluorescent dye in the tumor region, wherein the second near-infrared window collection module comprises a second near-infrared window camera configured to collect the second near-infrared window fluorescent images and the second near-infrared window white light images;
• a CT imaging module configured to irradiate the mouse and generate CT images for the mouse;
• and
• a central control module configured to reconstruct second near-infrared window three-dimensional and tomographic images and first near-infrared window three-dimensional and tomographic images based on the second near-infrared window white light images, the first near-infrared window white light images, the second near-infrared window fluorescent images, the first near-infrared window fluorescent images, and the CT images, wherein
• the second near-infrared window white light images and the first near-infrared window white light images are respectively used to register the second near-infrared window fluorescent images to the CT images and to register the first near-infrared window fluorescent images to the CT images;
• the second near-infrared window fluorescent images are used to reconstruct a second near-infrared window fluorescent light source distribution in a body of the mouse;
• and
• the first near-infrared window fluorescent images are used to reconstruct a first near-infrared window fluorescent light source distribution in the body of the mouse;
• wherein the central control module is configured to:
• after the white light emitting device is turned on, output control signals to the first near-infrared window camera and the second near-infrared window camera to cause the first near-infrared window camera and the second near-infrared window camera to collect the first near-infrared window white light images and the second near-infrared window white light images, respectively;
• after the white light emitting device is turned off and the laser is turned on to output the excitation light, output control signals to the first near-infrared window camera and the second near-infrared window camera to cause the first near-infrared window camera and the second near-infrared window camera to collect the first near-infrared window fluorescent images and the second near-infrared window fluorescent images, respectively;
• after the laser is turned off, output a control signal to the CT imaging module to collect the CT images;
• performing de-noising and enhancing pre-process on the collected first near-infrared window white light images, the collected second near-infrared window white light images, the collected first near-infrared window fluorescent images, the collected second near-infrared window fluorescent images, and the collected CT images;
• and
• registering the first near-infrared window fluorescent images and the second near-infrared window fluorescent images to the CT images respectively according to the first near-infrared window white light images and the second near-infrared window white light images, obtaining the three-dimensional images of the tumor in the body of the mouse by combining the second near-infrared window fluorescent light source distribution and the first near-infrared window fluorescent light source distribution, so as to achieve the second near-infrared window/first near-infrared window dual-mode fluorescence tomography reconstruction;
• wherein the system further comprises a rack, a turntable, and a mobile station, wherein:
• the rack comprises a first base and a second base,
• the turntable is mounted on the second base of the rack in a manner capable of rotating in a vertical plane, the turntable is configured to fix the lighting module, the excitation module, the second near-infrared window collection module, the first near-infrared window collection module and the CT imaging module, and a rotation of the turntable is driven by a power mechanism communicatively connected with the central control module;
• and
• the mobile station is configured to fix the mouse, the mouse is fixed to the mobile station by a mouse bed, the mobile station is movable in both a horizontal direction and a vertical direction, the mobile station comprises a vertical mobile station and a horizontal mobile station, the vertical mobile station is mounted on the horizontal mobile station in a manner capable of sliding in the vertical direction, and the horizontal mobile station is mounted, by parallel guides, on the first base of the rack in a manner capable of sliding in the horizontal direction;
• and
• wherein the second near-infrared window/first near-infrared window dual-mode fluorescence tomography method reconstructs second near-infrared window three-dimensional and tomographic images and first near-infrared window three-dimensional and tomographic images based on Gaussian weighted neighborhood fused Lasso regularization.