Analyte sensors employing multiple enzymes and methods associated therewith

A method comprising:

• performing a dip coating operation to deposit (i) a bilayer membrane upon a first active area disposed on a first working electrode of an analyte sensor, the first active area comprising an enzyme responsive to a first analyte, and (ii) a homogeneous membrane upon a second active area disposed on a second working electrode of the analyte sensor, the second active area comprising an enzyme responsive to a second analyte;
• wherein the first active area and the second active area are laterally spaced apart from one another;
• wherein the first active area and the second active area are responsive to different analytes:
• wherein the bilayer membrane comprises an inner layer comprising a first membrane polymer and an outer layer comprising a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another;
• wherein the homogeneous membrane comprises one of the first membrane polymer or the second membrane polymer;
• and
• wherein the inner layer of the bilayer membrane comprises a crosslinked polyvinylpyridine homopolymer or copolymer.

An analyte sensor comprising:

• at least a first working electrode and a second working electrode, a first active area disposed on the first working electrode and a second active area disposed on the second working electrode;
• wherein the first active area comprises an enzyme responsive to a first analyte and the second active area comprises an enzyme responsive to a second analyte;
• and
• wherein the first active area and the second active area are laterally spaced apart from one another;
• and
• a dip-coated bilayer membrane upon the first active area disposed on the first working electrode, and a dip-coated homogeneous membrane upon the second active area disposed on the second working electrode;
• wherein the bilayer membrane comprises an inner layer comprising a first membrane polymer and an outer layer comprising a second membrane polymer, the first membrane polymer and the second membrane polymer differing from one another;
• wherein the homogeneous membrane comprises one of the first membrane polymer or the second membrane polymer;
• and
• wherein the inner layer of the bilayer membrane comprises a crosslinked polyvinylpyridine homopolymer or copolymer.