US 12,004,494 B2 · Siglec transgenic mice and methods of use thereof

Abstract

Provided herein are transgenic non-human animals whose genomes comprise two or more human genes selected from CD33, Siglec-5, Siglec-7, Siglec-9, Siglec-11, Siglec-14, and Siglec-16, to methods of screening candidate agents that bind to and/or modulate the function and/or activity of at least one of the human genes in the transgenic non-human animals, and to methods of screening candidate agents to determine their effect on one or more activities and/or functions associated with expression of at least one of the human genes in the transgenic non-human animals. Further provided herein are methods of recapitulating a human Siglec immune system in a non-human animal, and methods of generating a non-human animal disease model comprising a human Siglec repertoire.

Claims22

Dependent claims

A method of screening candidate agents, the method comprising

i) administering one or more candidate agents to a transgenic mouse, wherein the genome of the transgenic mouse comprises two or more human genes, wherein the two or more human genes are selected from the group consisting of CD33, Siglec-5, Siglec-7, Siglec-9, Siglec-11, Siglec-14, and Siglec-16, wherein the two or more human genes are expressed in one or more cells of the transgenic mouse, and wherein the one or more cells are selected from the group consisting of myeloid cells, natural killer (NK) cells, T cells, microglia, and any combination thereof;
and
ii) determining whether the one or more candidate agents binds to and/or modulates the function and/or activity of at least one of the two or more human genes in the transgenic mouse.

Dependencies are inferred from the claim text.

Facts

Application

17/475,116Filed 14 Sep 2021 · division

Priority

8 Dec 2016

Published

US 2022/0000083 A16 Jan 2022

Granted

11 Jun 2024

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

A01K 67/0276
Genetically modified vertebrates, e.g. transgenic: knock-out vertebrates
A01K 67/0275
New or modified breeds of vertebrates: genetically modified vertebrates, e.g. transgenic
C07K 14/7056
Receptors; Cell surface antigens; Cell surface determinants: lectin superfamily, e.g. CD23, CD72
C07K 14/70596
Receptors; Cell surface antigens; Cell surface determinants: molecules with a "CD"-designation not provided for elsewhere

Family

Application62/431,661Us provisional application · filed 8 Dec 2016

Application15/836,089Parent of this division · filed 8 Dec 2017

US 2022/0000083 A1Pre-grant publication · published 6 Jan 2022

US 11,147,249 B2Parent of this division

US 2018/0160661 A1Published application

US 12,004,494 B2This patent

From the US filing history. Foreign family members aren’t listed.

Siglec transgenic mice and methods of use thereof

Life of this patent

Siglec transgenic mice and methods of use thereof

Life of this patent

Abstract

Claims22