Late-onset Alzheimer's disease animal model and uses thereof

An in vitro method for screening a test compound for its effect on amyloid β expression comprising the steps of:

• (a) providing a cell culture of primary brain cells cultured from a mouse whose expression of Shugoshin 1 (Sgo1) gene has been genetically modified to enable modulation of Sgo1 expression in the brain of the mouse when exposed to an Sgo1 expression-modulating compound, wherein the mouse is a Sgo1-CRISPR-cre-lox mutant mouse and the Sgo1 expression-modulating compound is tamoxifen, wherein the cells of the cell culture are induced to accumulate amyloid-β due to inhibition or reduction of Sgo1 expression when the Sgo1 expression-modulating compound is added to the cell culture;
• (b) contacting the culture of (a) with a test compound and tamoxifen,
• (c) assaying the culture of (b) for the presence or amount amyloid β and comparing the culture of (b) with a control culture contacted with tamoxifen but not contacted with the test compound, wherein a reduction of amyloid β expression as compared to the control culture indicates that the test compound is effective in reducing amyloid β expression.