Light synchronization for an imaging system
AssigneeAraceli Biosciences Inc.
InventorsMarguerite Vantangoli Policelli, Marshall Anderson, David Tasker, Tyler Wilson
Life of this patent
Estimating the term…
Abstract
Methods and systems are provided for synchronizing image capture at a multi-detector imaging system. In one example, a method includes coordinating cycling of each microscope assembly of the multi-detector imaging system through a selection of illumination channels, each microscope assembly configured to obtain an image of a portion of one of more than one microplate wells simultaneously, to generate complete images of the more than one microplate wells concurrently.
Claims20
A method for a multi-detector imaging system, comprising:
- receiving an input from a user at a graphical user interface, the input including imaging protocols and a microplate type;
- determining parameters of a microplate based on the microplate type;
- auto-focusing an objective of each of a plurality of microscope assemblies of the multi-detector imaging system at the microplate;
- and
- coordinating image acquisition amongst the plurality of microscope assemblies according to the imaging protocols input by the user to acquire complete images of wells of the microplate.
A multi-detector imaging system comprising:
- two or more microscope assemblies having objectives arranged at tops of plates oriented with heights of the plates parallel with a central axis of the multi-detector imaging system, tube lenses coupled to the plates and positioned below the objectives, laser auto-focuses coupled to a central region of the plates and oriented parallel with the central axis, and light sources located between the tube lenses and the laser auto-focuses;
- a microplate positioned over the objectives;
- and
- a controller with computer readable instructions stored on non-transitory memory that, when executed, cause the controller to:
- collect images of the microplate at a same light frequency output from the light sources of the two or more microscope assemblies;
- and
- compile complete images of wells of the microplate from the collected images and display the complete images at a graphical user interface.
A method for synchronous image capture at a multi-detector imaging system, comprising:
- acquiring images concurrently at quadrants of a microplate from microscope assemblies of the multi-detector imaging system by simultaneously illuminating the quadrants of the microplate with a first frequency of light,
- wherein the microscope assemblies are supported on plates oriented with heights of the plates parallel with a central axis of the multi-detector imaging system and in an x-shaped configuration, and
- wherein objectives are positioned at tops of the plates, tube lenses are located below the objectives, and laser auto-focus sensors are coupled to central regions of the plates and oriented parallel with the heights of the plates.
Dependencies are inferred from the claim text.