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US 12,000,835 B2Granted
Methods and compositions for protein sequencing
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Jeremy Lackey, Haidong Huang
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Abstract
Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules having a shielding element that enhances photostability in polypeptide sequencing reactions.
Claims29
A method of identifying an amino acid of a polypeptide, the method comprising:
- contacting a single polypeptide molecule with one or more amino acid recognition molecules that bind to the single polypeptide molecule, wherein at least one amino acid recognition molecule comprises an N-end rule pathway protein;
- detecting a series of signal pulses indicative of association of the one or more amino acid recognition molecules with the single polypeptide molecule under polypeptide degradation conditions in a reaction mixture comprising a first aminopeptidase and a second aminopeptidase,
- wherein the first aminopeptidase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:
- 48, and the second aminopeptidase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:
- 59 or 69;
- and
- identifying a first type of amino acid in the single polypeptide molecule based on a first characteristic pattern in the series of signal pulses.
4 more elements
Dependencies are inferred from the claim text.