Method of selecting stem cells having ability to produce extracellular vesicles with high efficiency using activation of protease-activated receptor-mediated signaling pathways

A method of selecting a stem cell having an ability to produce extracellular vesicles with high efficiency, the method comprising measuring an activation level of a protease-activated receptor (PAR)-mediated signaling pathway,

• wherein the PAR is PAR1 or PAR3,
• wherein the ability to produce extracellular vesicles with high efficiency is promotion of the production of extracellular vesicles or promotion of a level of a protein in extracellular vesicles, and
• wherein the measurement of the activation level of the PAR-mediated signaling pathway comprises the following processes:
• (a) culturing stem cells and then treating the stem cells with thrombin;
• (b) measuring an expression level of one or more selected from the group consisting of Ras-related protein Rab-5 (Rab-5), early endosome antigen-1 (EEA-1), phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2), and phospho-protein kinase B (p-AKT) in the thrombin-treated stem cells;
• and
• (c) selecting a stem cell in which the expression level has been increased, as a stem cell having an ability to produce extracellular vesicles with high efficiency.