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US 12,000,805 B2Granted
Method of introducing a sample into a separation column and corresponding system
AssigneeDionex Softron GmbH
InventorsChristoph Hollnagel, Hermann Hochgraeber, Thomas Wachinger
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Abstract
A method of introducing a sample into a separation column includes introducing the sample into a trap column, isolating the trap column from ambient atmosphere and pressurizing the trap column to a first pressure while the trap column is isolated from ambient atmosphere, providing a fluid connection between the trap column and the separation column after pressurizing the trap column to the first pressure, supplying the sample from the trap column to the separation column.
Claims12
A liquid chromatography system comprising
- a sample pick up means,
- a metering device configured to be fluidly connected to the sample pick up means;
- a seat configured to receive the sample pick up means;
- a trap column;
- a separation column;
- an analytical pump;
- a first distributor valve comprising:
- a plurality of ports;
- and
- a plurality of connecting elements configured to changeably connect to the plurality of ports of the first distributor valve, wherein the plurality of ports of the first distributor valve comprises:
- a first port directly fluidly connected to the seat;
- a second port and a third port that are both directly fluidly connected to the trap column;
- a fourth port directly fluidly connected to the separation column;
- a fifth port directly fluidly connected to the analytical pump;
- wherein the liquid chromatography system is configured to isolate the trap column from an ambient atmosphere and to pressurize the trap column to a pressure exceeding the ambient pressure without the trap column being fluidly connected to the analytical pump.
12 more elements
Dependencies are inferred from the claim text.