System suitability method for use with protein concentration determination by slope
AssigneeAmgen Inc.
InventorsRobert John Duff, Christian Gutierrez
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Abstract
Disclosed are methods of determining the suitability of a variable-length spectrophotometer using Patent Blue dye or AMG Blue dye. Also disclosed herein are methods of determining the suitability of a fixed path length spectrophotometer for determining protein concentration of a protein sample. AMG Blue dye may also be used to determine the suitability of fixed path length spectrophotometers.
Claims17
A method of determining the suitability of a variable-length spectrophotometer for determining protein concentration of a protein sample, comprising measuring the absorbance of Patent Blue dye (PBD) or AMG Blue Dye (ABD) at least two wavelengths, the first wavelength of 280 nm and the subsequent wavelength(s) selected from the group consisting of 310 nm, 412 nm, 510 nm and 615 nm.
A method of determining the suitability of a fixed path length spectrophotometer for determining protein concentration of a protein sample, comprising measuring the absorbance of ABD at least two wavelengths, the first wavelength of 280 nm and the subsequent wavelength(s) selected from the group consisting of 310 nm, 412 nm, 510 nm and 615 nm.
Dependencies are inferred from the claim text.