Modification of 5-methylcytosine catalyzed by Cmd1 enzyme and application thereof
AssigneeCenter for Excellence In Molecular Cell Science, Chinese Academy of Sciences
InventorGuoliang Xu
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Abstract
The present invention relates to a novel modification of a 5-methylcytosine nucleic acid catalyzed by a Cmd1 enzyme and an application thereof. The present inventors have for the first time discovered Cmd1, a 5mC-modifying enzyme, which can link a glyceryl group to a 5mC methyl carbon of a methylated nucleic acid through a carbon-carbon single bond, and this is a new epigenetic modification.
Claims11
A method for adding a glyceryl group to a methyl group of 5-methylcytosine of a methylated nucleic acid, comprising:
- treating the methylated nucleic acid with a Cmd1 enzyme, so that the glyceryl group is added to the methyl group of 5-methylcytosine of the methylated nucleic acid;
- the Cmd1 enzyme comprises:
- (a) a polypeptide of an amino acid sequence of SEQ ID NO:
- 1;
- (b) a polypeptide derived from (a), being formed by subjecting the amino acid sequence of SEQ ID NO:
- 1 to substitution, deletion or addition of one or more amino acid residues, and having a function of the polypeptide of (a);
- or (c) a polypeptide derived from (a), having more than 60% homology to the polypeptide sequence defined in (a) and having a function of the polypeptide of (a);
- and in the sequences defined in (b) and (c), based on the amino acid sequence of SEQ ID NO:
- 1, the 330th position is A, the 345th position is H, the 347th position is D, and the 350th position is D.
A method for adding a glyceryl group to a methyl group of 5-methylcytosine of a methylated nucleic acid, comprising:
- treating the methylated nucleic acid with an up-regulator of a Cmd1 enzyme, so that the glyceryl group is added to the methyl group of 5-methylcytosine of the methylated nucleic acid;
- the Cmd1 enzyme comprises:
- (a) a polypeptide of an amino acid sequence of SEQ ID NO:
- 1;
- (b) a polypeptide derived from (a), being formed by subjecting the amino acid sequence of SEQ ID NO:
- 1 to substitution, deletion or addition of one or more amino acid residues, and having a function of the polypeptide of (a);
- or (c) a polypeptide derived from (a), having more than 60% homology to the polypeptide sequence defined in (a) and having a function of the polypeptide of (a);
- and in the sequences defined in (b) and (c), based on the amino acid sequence of SEQ ID NO:
- 1, the 330th position is A, the 345th position is H, the 347th position is D, and the 350th position is D;
- the up-regulator of the Cmd1 enzyme is an expression vector expressing the Cmd1 enzyme.
Dependencies are inferred from the claim text.