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US 11,999,992 B2Granted
Nucleic acid stabilization reagent, kits, and methods of use thereof
AssigneeBruker Cellular Analysis, Inc.
InventorJason M. McEwen
Life of this patent
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Abstract
Reagents for stabilizing the nucleic acids of a biological cell, compositions, kits and methods of use thereof are described. The stabilization reagents may prepare the nucleic acids within the biological cell for storage and preserve the representative population of the nucleic acids for later isolation and analysis.
Claims21
A method of stabilizing a population of ribonucleic acids in a biological cell, wherein the biological cell is an animal cell, comprising:
- contacting the animal cell with at least one irreversible protein translation inhibitor;
- at least one ribonucleic acid transcription inhibitor;
- and at least one electron transport chain agent comprising an electron transport chain inhibitor and/or an electron transport chain decoupling agent, wherein the at least one ribonucleic acid transcription inhibitor is a CDK9 inhibitor, aurethricin, thiolutin, amanitin, and/or triptolide;
- and/or the electron transport chain agent is rotenone, 2-thenoyltrifluoroacetone, carboxin, cyanide, sodium azide, 2,4 dinitrophenol, dicumarol, and/or carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone;
- and
- wherein the contacting is performed for a period of time sufficient to retain the size distribution of the population of ribonucleic acids within the animal cell.
3 more elements
A method of stabilizing a population of ribonucleic acids in a biological cell, wherein the biological cell is an animal cell, comprising:
- contacting the animal cell with at least one irreversible protein translation inhibitor;
- at least one ribonucleic acid transcription inhibitor;
- and at least one electron transport chain agent comprising an electron transport chain inhibitor and/or an electron transport chain decoupling agent, wherein the at least one ribonucleic acid transcription inhibitor is a CDK9 inhibitor, aurethricin, thiolutin, amanitin, and/or triptolide;
- and/or the electron transport chain agent is rotenone, 2-thenoyltrifluoroacetone, carboxin, cyanide, sodium azide, 2,4 dinitrophenol, dicumarol, and/or carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone;
- and
- wherein the contacting is performed for a period of time sufficient to disrupt cellular processes for production and degradation of ribonucleic acids in the population of ribonucleic acids within the animal cell.
3 more elements
Dependencies are inferred from the claim text.