Method for assaying the activity of spermidine/spermine N1-acetyltransferase
AssigneeBioMark Technologies Inc.
InventorsBrian Cheng, Rashid Bux, Daniel Sitar
Life of this patent
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Abstract
A method for assaying activity of the enzyme spermidine/spermine N1-acetyltransferase (SSAT) uses SSAT substrates by detecting their acetylated forms. SSAT substrates may include rimantadine and tocainide wherein their metabolism occurs in part by the action of the inducible enzyme SSAT to produce the acetylated metabolites N-acetylrimantadine and N-acetyltocainide respectively. SSAT activity may be correlated to pathologic conditions.
Claims8
A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
- (a) providing a biological sample from the mammal obtained after an administration of a dose of rimantadine, wherein the biological sample comprises a detectable level of N-acetyl rimantadine;
- and
- (b) quantifying the detectable level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.
A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
- (a) administering a dose of rimantadine to the mammal;
- (b) collecting a biological sample from the mammal following the administration of the dose of rimantadine, wherein the biological sample has a detectable level of N-acetyl rimantadine;
- and
- (c) quantifying the detectable level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.
Dependencies are inferred from the claim text.