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US 11,999,979 B2Granted
Lachnospiraceae sp. CAS12A mutants with enhanced cleavage activity at non-canonical TTTT protospacer adjacent motifs
AssigneeIntegrated Dna Technologies, Inc.
InventorsLiyang Zhang, Christopher Anthony Vakulskas
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Abstract
Described herein are CAS12A mutants from Lachnospiraceae bacterium and methods for use thereof. These mutants have enhanced DNA cleavage activities at non-canonical TTTT protospacer adjacent motifs (PAM) compared to the wild-type enzyme.
Claims2
An isolated mutant Lachnospiraceae bacterium ND2006 Cas12a (“LbCas12a”) polypeptide comprising the G146R/R182V/E795Q polypeptide sequence of SEQ ID NO:
- 39844.
Dependencies are inferred from the claim text.