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US 11,999,977 B2Granted
In situ enzymatic degradation of hydrocarbon-polluted soils
AssigneeInstitut National De La Recherche Scientifique
InventorsSatinder Kaur Brar, Tayssir Guedri, Tarek Rouissi
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Abstract
The present invention provides crude enzyme extracts, cocktails and compositions from Alkanivorax borkumensis and methods for the enzymatic treatment and bioremediation of petroleum hydrocarbon polluted ecosystems.
Claims23
A composition comprising at least one selected from the group consisting of:
- i) a cell free supernatant of Alkanivorax borkumensis (A. borkumensis) comprising an extracellular enzyme extract;
- ii) a supernatant of sonicated cells of A. borkumensis comprising an intracellular enzyme extract and an extracellular enzyme extract;
- and
- iii) combinations thereof;
- and
- between about 0.5% (v/v) and about 12% (v/v) of petroleum-derived hydrocarbons;
- wherein the composition comprises a plurality of polypeptides that exhibit at least two enzymatic activities selected from the group consisting of lipase activity, esterase activity, and alkane hydroxylase activity.
4 more elements
Dependencies are inferred from the claim text.