Loading patent…
AssigneeUniversity of Kentucky Research Foundation
InventorsGeorge Wagner, Antoaneta Borissova Mahaylova-Kroumova, Guiliang Tang, Erming Wang
Estimating the term…
The present invention provides a method for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, the method comprising modifying said tobacco plant or tobacco cell culture by inhibiting the activity or expression of a diterpene synthesis gene. The present invention also provides for the use of a diterpene synthesis gene for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, as well as tobacco cells, tobacco plants, tobacco plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.
A method for producing a tobacco plant or part thereof, a tobacco plant propagation material, a tobacco leaf, a cut harvested tobacco leaf, a processed tobacco leaf or a cut and processed tobacco leaf or a tobacco cell culture or tobacco product which has increased sucrose ester content, the method comprising modifying said tobacco to inhibit the activity or expression of a diterpene synthesis gene, wherein the sucrose ester content is increased in comparison to a tobacco plant or a tobacco cell culture which has not been modified to inhibit the activity or expression of a diterpene synthesis gene, and wherein
A tobacco plant or part thereof or a tobacco cell culture which has been modified to achieve an increase in sucrose ester content in comparison to an unmodified plant or an unmodified tobacco cell culture, wherein the modification is the inhibition of the activity or expression of a diterpene synthesis gene, wherein
A harvested leaf of a tobacco plant modified to achieve an increase in sucrose ester content in comparison to an unmodified tobacco leaf, wherein the modification is the inhibition of the activity or expression of a diterpene synthesis gene, wherein
A processed tobacco leaf of a tobacco plant, modified to achieve an increase in sucrose ester content in comparison to an unmodified tobacco leaf, where the modification is the inhibition of the activity or expression of a diterpene synthesis gene, wherein the diterpene synthesis gene is a terpene synthase3-8 gene having a polynucleotide sequence as set forth in SEQ ID No. 3, or a polynucleotide sequence with at least 90% sequence identity thereto, or having a polynucleotide sequence encoding a polypeptide comprising the amino acid sequence as set forth in SEQ ID No. 4 or a polynucleotide sequence with at least 90% sequence identity thereto.
A processed tobacco leaf of a tobacco plant modified to achieve an increase in sucrose ester content in comparison to an unmodified tobacco leaf, where the modification is the inhibition of the activity or expression of a diterpene synthesis gene,
A tobacco industry product prepared from a tobacco plant, part thereof, plant propagation material thereof, selected from a harvested leaf, processed tobacco leaf, cured tobacco material or a tobacco blend comprising said cured tobacco modified to achieve an increase in sucrose ester content in comparison to an unmodified tobacco plant or part thereof, wherein the modification is the inhibition of the activity or expression of a diterpene synthesis gene, wherein the diterpene synthesis gene is selected from:
A method for producing a tobacco industry product or cured tobacco leaf which has increased sucrose ester content, the method comprising producing a tobacco plant, wherein said tobacco plant is modified to inhibit the activity or expression of a diterpene synthesis gene, wherein the sucrose ester content is increased in comparison to a tobacco plant or a tobacco cell culture which has not been modified to inhibit the activity or expression of a diterpene synthesis gene and wherein the diterpene synthesis gene is selected from:
Dependencies are inferred from the claim text.