Back to results
US 11,999,958 B2Granted
Methods of gene editing and transforming Cannabis
AssigneeWisconsin Alumni Research Foundation
InventorsMichael W. Petersen, Edward James Williams, Robert Harnish, Heidi Flewelling Kaeppler, Brian Martinell, Ray Collier, Frank McFarland, Shawn Michael Kaeppler
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods for the production of Cannabis meristem explants from dry seeds. Also described are methods of transforming and gene editing using the Cannabis meristem explants disclosed herein.
Claims11
A method of transforming a Cannabis plant, the method comprising:
- growing a sanitized and imbibed Cannabis seed on a non-selective culture medium suitable for supporting the growth and survival of the Cannabis seed;
- harvesting an internode, leaf, or petiole tissue to form a Cannabis explant;
- inoculating the Cannabis explant with a heterologous nucleic acid;
- co-culturing the Cannabis explant in a co-culture medium for between about 1 day and about 6 days,
- culturing the Cannabis explant on a selection medium comprising meta-topolin to select for transformed Cannabis explants.
2 more elements
Dependencies are inferred from the claim text.