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AssigneeQilu University of Technology
InventorsXiaoming Bao, Liyun Song, Xinning Wang, Wenyan Cao, Weiquan Zhao, Zailu Li
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A method of enhancing vanillin resistance of Saccharomyces cerevisiae, including: knocking out SNG1 gene from a genome of Saccharomyces cerevisiae. This application further provides a mutant of SNG1 gene of Saccharomyces cerevisiae including the nucleotide sequence shown in SEQ ID NO: 1, where the sequence shown in SEQ ID NO: 1, from left to right, consists of a −18˜+203 bp fragment of SNG1 gene of Saccharomyces cerevisiae, a nucleotide fragment of loxp-KanMX4-loxp and a +1446˜+1644 bp fragment of the SNG1 gene of Saccharomyces cerevisiae.
A mutant SNG1 gene, comprising the nucleotide sequence of SEQ ID NO:
A Saccharomyces cerevisiae cell with vanillin resistance, the Saccharomyces cerevisiae cell comprising a mutant SNG1 gene comprising the nucleotide sequence of SEQ ID NO:
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