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US 11,999,951 B2Granted
Massively parallel contiguity mapping
AssigneeUniversity of Washington through its Center for Commercialization
InventorsJay Ashok Shendure, Jerrod Joseph Schwartz, Andrew Colin Adey, Cho li Lee, Joseph Brian Hiatt, Jacob Otto Kitzman, Akash Kumar
Life of this patent
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Abstract
Contiguity information is important to achieving high-quality de novo assembly of mammalian genomes and the haplotype-resolved resequencing of human genomes. The methods described herein pursue cost-effective, massively parallel capture of contiguity information at different scales.
Claims14
A method for preparing a sequencing library, comprising:
- (a) contacting a target DNA molecule with a transposase, resulting in multiple insertions of a continuous transposon to produce a modified target DNA molecule, wherein the continuous transposon comprises an adaptor domain;
- (b) amplifying fragments of the target DNA molecule by contacting the modified target DNA molecule with oligonucleotide primers that anneal to the adaptor domain, wherein each oligonucleotide primer comprises the same compartment-specific barcode sequence;
- and
- (c) before step (b), performing one of the following:
- (i) prior to step (a), compartmentalizing the target DNA molecule, or
- (ii) after step (a), compartmentalizing the modified target DNA molecule;
- wherein steps (a), (b), and (c) create a plurality of tagged target DNA fragments each comprising an identical or complementary barcode sequence.
4 more elements
Dependencies are inferred from the claim text.