Process and system for propagating cell cultures while preventing lactate accumulation

A process for propagating a cell culture comprising:

• determining a concentration of at least one quality attribute in a cell culture;
• measuring values of at least two attribute influencing parameters within the cell culture;
• sending the concentration of the at least one quality attribute and the values of the attribute influencing parameter measurements to a controller, the controller including a predictive model that is configured to calculate a future concentration of the at least one quality attribute in the cell culture based at least in part on the values of the attribute influencing parameters;
• running simulations on an optimizer to calculate future concentrations of the at least one quality attribute based on manipulation of the attribute influencing parameters within the cell culture;
• and
• the controller selectively changing at least one condition within the cell culture based upon the determined future concentration of the at least one quality attribute in the cell culture for maintaining the at least one quality attribute concentration within preset limits.