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US 11,999,767 B2Granted
Target recognition motifs and uses thereof
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology
InventorFeng Zhang
Life of this patent
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Abstract
The disclosure provides novel programmable targeting sequences and applications thereof. The targeting sequences can be engineered for binding to proteins, polypeptides, and other macromolecules.
Claims7
A method of engineering a protein or polypeptide to bind to a target of interest, which comprises:
- inserting one or more engineered, heterologous Photorhabdus tandem repeat protein target recognition sequence (TRS) motif(s) in the protein or polypeptide, and modifying the one or more engineered, heterologous Photorhabdus TRS motif(s);
- associating the one or more modified, engineered, heterologous Photorhabdus TRS motif(s) with a detectable marker;
- and
- detecting whether the engineered protein or polypeptide binds to the target;
- or
- detecting whether the engineered protein or polypeptide exhibits enzymatic activity against the target.
3 more elements
Dependencies are inferred from the claim text.