Method for continuously collecting fluorescence data of microfluidic chip

A method for continuously collecting fluorescence data of a microfluidic chip, comprising:

• emitting, by an optical path system, a light perpendicular to the microfluidic chip, wherein centers of all reaction cells forms a same circle, and a center of a light spot formed by the light on the microfluidic chip is always located on the circle formed by the centers of all reaction cells;
• rotating the microfluidic chip around a center of the circle formed by the centers of all the reaction cells, and collecting fluorescence signal values along a rotation direction of the microfluidic chip by using the optical path system;
• and
• processing the collected fluorescence signal values to obtain effective fluorescence data of all the reaction cells,
• wherein the microfluidic chip is rotated by one revolution to collect fluorescence signal values of all the reaction cells for each time,
• wherein the processing the collected fluorescence signal values to obtain effective fluorescence data of all the reaction cells comprises:
• step (1), determining a maximum value A among fluorescence signal values of one of the reaction cell;
• step (2), calculating B according to B=A*S, where S represents a preset coefficient, and 0<S<1:
• step (3), counting the number N of fluorescence signal values greater than B among the fluorescence signal values of the reaction cell;
• step (4), determining A as the effective fluorescence data of the reaction cell if N is greater than or equal to P, where P represents a preset threshold,
• wherein the steps (1) to (4) are performed for each of the reaction cells to obtain effective fluorescence data of each of the reaction cells.