Antibody-drug conjugates containing an anti-mesothelin antibody and uses thereof

An immunoconjugate, comprising:

• an antibody comprising an antigen-binding fragment that specifically binds to an epitope in mesothelin, an N-glycan binding domain and an N-glycan having a structure of formula (1);
• wherein “*” represents a bond or a protecting group;
• a linker linking to each of the “*” in the N-glycan when “*” presents the bond;
• and
• a payload A and a payload B independently conjugated to the linkers;
• wherein the payload A and the payload B are the same or different;
• wherein the antibody comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:
• 11, and a light chain variable region comprising the amino acid sequence of SEQ ID NO:
• 12.