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US 11,998,567 B2Granted
Engineered chimeric guide RNA and uses thereof
AssigneeNanjing Bioheng Biotech Co., Ltd.
InventorsJiangtao Ren, Xuhua Zhang
Life of this patent
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Abstract
The present invention relates to engineered chimeric guide RNAs and their uses in systems and methods for gene expression perturbation in eukaryotic cells. Furthermore, the invention also relates to methods for generating a modified T cell using the engineered chimeric guide RNAs of the present application, and to the obtained modified T cell. Also included are methods and pharmaceutical compositions comprising the modified T cell for adoptive therapy and treating a condition, such as cancer, infections or autoimmune diseases.
Claims4
An engineered chimeric guide RNA comprising:
- (1) at least one crRNA sequences, and
- (2) at least one additional direct repeat sequences and small RNA species,
- wherein the crRNA sequence is capable of hybridizing with a target locus of interest, and the additional direct repeat sequence and/or small RNA species confers increased stability to the chimeric guide RNA;
- wherein the small RNA species are a truncated form of pre-tRNA.
1 more element
Dependencies are inferred from the claim text.