Interference rejection membranes useful with analyte sensors

A method of making an amperometric analyte sensor for implantation within a mammal comprising the steps of:

• providing a first base layer;
• forming a conductive layer on the first base layer, wherein the conductive layer includes a first working electrode;
• forming an analyte sensing layer on the conductive layer, wherein the analyte sensing layer includes an enzyme selected to generate a detectable electrical signal upon exposure to an analyte, wherein:
• the analyte sensing layer is formed by combining a polymerizable monomer, a crosslinking agent and a photoinitiator agent;
• forming an interference rejection layer on the analyte sensing layer, wherein:
• the interference rejection layer is formed by a reaction mixture comprising a polymerizable monomer, a crosslinking agent and a photoinitiator agent;
• the reaction mixture comprises a 15% to 96% of crosslinker to monomer ratio;
• and
• the analyte sensing layer and the interference rejection layer are formed when the reaction mixture is polymerized by exposure to light;
• and
• forming an analyte modulating layer on the interference rejection layer;
• such that an amperometric analyte sensor for implantation within a mammal is made.