Microbial fermentation methods and compositions
AssigneeNewLeaf Symbiotics, Inc.
InventorGregg Bogosian
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Abstract
The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.
Claims37
A method for treating a plant or a plant part with Methylobacterium comprising the step of applying to said plant or plant part a composition comprising a fermentation product comprising a solid substance wherein a mono-culture or co-culture of Methylobacterium is adhered thereto by having been grown on said solid substance, wherein said solid substance comprises a plurality of particles of about 1 micron to about 1000 microns in average length or average diameter and is not a photosynthetic microorganism, and wherein said fermentation product is essentially free of contaminating microorganisms other than the Methylobacterium, and wherein said composition (i) has a Methylobacterium titer of at least 5×108 colony-forming units per ml of said composition for a fermentation broth, or (ii) has a Methylobacterium titer of at least 5×108 colony-forming units per gram of said composition for a dry composition.
A plant or plant part that is at least partially coated with a composition comprising a fermentation product that comprises:
- (i) an exogenous solid substance having a mono-culture or co-culture of Methylobacterium adhered thereto by having been grown on said solid substance as an axenic culture that is essentially free of contaminating microorganisms;
- and (ii) an agriculturally acceptable adjuvant, wherein said adjuvant is a sticker, extender, humectant, drift-reducer, water conditioner, coating promoter, or combination thereof;
- an agriculturally acceptable excipient;
- or a combination thereof.
Dependencies are inferred from the claim text.