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US 11,998,002 B2Granted
Cryopreserving processes
AssigneeUniversity of Warwick
InventorsMatthew Gibson, Christopher Stubbs, Trisha Bailey
Life of this patent
Estimating the term…
Abstract
The present invention relates to processes for producing compositions for the cryopreservation of biological materials, e.g. cells and proteins. The compositions comprise a polyampholyte polymer. The invention also provides certain cryopreserving compositions comprising the polyampholyte polymer.
Claims13
A process for producing a cryopreserved composition comprising biological material, the process comprising the step:
- (a) freezing biological material at a cryopreserving temperature in a cryopreserving composition comprising a compound of Formula I:
- wherein n is 20-5000,
- wherein the biological material is selected from the group consisting of cells, monolayers of cells and spheroids.
A cryopreserving composition comprising a compound of Formula I,
- wherein n is 20-5000,
- wherein the concentration of the compound of Formula I in the composition is 1-15 mg/ml, 25-150 mg/ml, 50-200 mg/ml, 75-150 mg/ml, or about 100 mg/ml.
A cryopreserving composition comprising a compound of Formula I
- wherein n is 20-5000,
- wherein the cryopreserving composition additionally comprises:
- (a) a physiologically-acceptable buffer;
- or
- (b) biological material, wherein the biological material is selected from the group consisting of cells, monolayers of cells and spheroids.
2 more elements
A kit comprising:
- (a) a compound of Formula I,
- wherein n is 20-5000,
- together with
- (b) one or more components selected from the group consisting of an aqueous buffer, an antibiotic, a sugar, an anticoagulant, an antioxidant, a pH indicator, glycerol and DMSO.
1 more element
Dependencies are inferred from the claim text.