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US 11,997,994 B2Granted
Genetically modified non-human animals with common light chain immunoglobulin locus
AssigneeBiocytogen Pharmaceuticals (Beijing) Co., Ltd.
InventorsYabo Zhang, Hui Lu, Huizhen Zhao, Jiawei Yao, Yi Yang, Yuelei Shen
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Abstract
This disclosure relates to genetically modified animals and cells with humanized light chain immunoglobulin locus and/or humanized heavy chain immunoglobulin locus. In one aspect, the endogenous light chain immunoglobulin locus comprises a limit number of human IGKV genes and human IGKJ genes.
Claims18
A genetically-modified mouse whose genome comprises at the endogenous kappa light chain immunoglobulin locus, an exogenous kappa light chain variable region gene sequence,
- wherein the exogenous kappa light chain variable region gene sequence comprises only one human IGKV gene and only one human IGKJ gene,
- wherein the human IGKV gene and the one human IGKJ gene are operably linked to an endogenous light chain constant domain gene, and the endogenous kappa light chain variable region gene sequence is nonfunctional, wherein
- (1) the human IGKV gene is IGKV 1-39 and the human IGKJ gene is IGKJ4;
- or
- (2) the human IGKV gene is IGKV 3-11 and the human IGKJ gene is IGKJ1,
- wherein the mouse produces an antibody comprising a human light chain variable region (VL) in response to an antigen.
3 more elements
Dependencies are inferred from the claim text.