Membrane protein analysis substrate, method of producing membrane protein analysis substrate, method of analyzing membrane protein and membrane protein analysis grid
AssigneeKyushu University, National University Corporation
InventorAtsushi Shimada
Life of this patent
Estimating the term…
Abstract
A membrane protein analysis substrate including an electron microscope grid having a plurality of through-holes; a lipid bilayer membrane that is provided to cover at least one of the plurality of through-holes; and membrane proteins that are retained in a part planarly overlapping the through-holes of the lipid bilayer membrane, wherein the lipid bilayer membrane has a lipid monolayer, and wherein the lipid monolayer is larger than the through hole in a plan view, adheres to the grid, and constitutes a part of the lipid bilayer membrane.
Claims20
A membrane protein analysis substrate, comprising:
- an electron microscope grid having a plurality of through-holes;
- a lipid bilayer membrane that is provided to cover at least one of the plurality of through-holes;
- and
- membrane proteins that are retained in a part planarly overlapping positions of the through-holes covered by the lipid bilayer membrane,
- wherein the lipid bilayer membrane has a lipid monolayer, and
- wherein the lipid monolayer is larger than the through-hole in a plan view, adheres to the electron microscope grid, and constitutes a part of the lipid bilayer membrane.
A method of forming a lipid bilayer membrane of producing a membrane protein analysis substrate, comprising:
- a process of providing a first lipid monolayer on a first surface of an electron microscope grid having a plurality of through-holes;
- a process of providing a second lipid monolayer on a second surface of the electron microscope grid and forming a lipid bilayer membrane in which the first lipid monolayer and the second lipid monolayer are bonded;
- and
- a process of retaining membrane proteins in the lipid bilayer membrane;
- wherein, in the process of providing a first lipid monolayer, the plurality of through-holes are covered from the side of the first surface with the first lipid monolayer,
- wherein, in the process of forming a lipid bilayer membrane, the plurality of through-holes are covered from the side of the second surface with the second lipid monolayer, and
- wherein the first lipid monolayer and the second lipid monolayer are bonded inside the plurality of through-holes.
A method of producing a membrane protein analysis substrate, comprising:
- a process of bringing one surface of an electron microscope grid having a plurality of through-holes into contact with a lipid monolayer formed on a liquid surface of a solution and forming a lipid bilayer membrane inside the plurality of through-holes;
- and
- a process of adding membrane proteins to the solution and retaining the membrane proteins in the lipid bilayer membrane,
- wherein, in the process of forming a lipid bilayer membrane, the lipid monolayer is transferred to the one surface, and the plurality of through-holes are covered from the side of the one surface with the lipid monolayer,
- wherein the lipid monolayer forms a self-assembled lipid bilayer membrane inside the plurality of through-holes, and
- wherein, in the process of retaining membrane proteins, the membrane proteins move from the solution to the lipid bilayer membrane and are retained in the lipid bilayer membrane.
A method of producing a membrane protein analysis substrate, comprising:
- a process of forming a lipid bilayer membrane retaining membrane proteins;
- and
- a process of transferring the lipid bilayer membrane retaining the membrane protein to one surface of an electron microscope grid having a plurality of through-holes,
- wherein, in the process of transferring to one surface, the lipid bilayer membrane is brought into contact with the one surface in such a manner that it covers the entire through-holes.
A membrane protein analysis grid, comprising:
- an electron microscope grid having a plurality of through-holes;
- and
- a lipid bilayer membrane that is provided to cover at least one of the plurality of through-holes,
- wherein one lipid monolayer constituting the lipid bilayer membrane covers a first surface of the electron microscope grid,
- wherein the other lipid monolayer constituting the lipid bilayer membrane covers a second surface of the electron microscope grid, and
- wherein the one lipid monolayer and the other lipid monolayer are bonded inside the through-hole, form the lipid bilayer membrane, and cover the through-hole.
Dependencies are inferred from the claim text.