Nanopore sensor calibration and operation with a fluidic passage

A method for calibrating a nanopore sensor comprising:

• providing a first fluidic solution, having a first ionic concentration, in a first fluidic reservoir, said first fluidic reservoir being disposed in fluidic connection with an inlet to a nanopore;
• providing a second fluidic solution, having a second ionic concentration, in a second fluidic reservoir, said second fluidic reservoir being disposed in fluidic connection with an outlet of said nanopore through a fluidic passage having at least one fluidic section with a fluidic section length greater than a fluidic section width and a passage width greater than a diameter of the nanopore;
• measuring an electrical potential local to the fluidic passage;
• determining a fluidic passage fluidic resistance, RFP, and a nanopore fluidic resistance, RPore based on measured fluidic passage electrical potential;
• comparing the fluidic passage fluidic resistance, RFP, with the nanopore fluidic resistance, RPore;
• adjusting at least one of the first ionic concentration and the second ionic concentration based on said comparing;
• and
• carrying out the measuring, determining, comparing, and adjusting steps until the fluidic passage fluidic resistance, RFP, is between about 0.1 times and about 10 times the nanopore fluidic resistance, RPore.