Arrangement and method for PCR with multi-channel fluorescence measurement for spatially distributed samples

An arrangement for polymerase chain reaction with multi-channel fluorescence measurement, the arrangement comprising:

• two heating elements, each including a sample receptacle configured to receive one sample carrier, wherein the sample carriers each include a plurality of cavities arranged in rows and columns;
• two measuring heads, which are each movable relative to the heating elements, wherein each measuring head is configured such that excitation light is coupled into each cavity in a column of the respective sample carrier and fluorescence light is coupled out from each cavity in the column;
• a fluorescence unit, comprising:
• a rotatable carrier;
• at least one optical module disposed at least partially on the carrier and configured to generate excitation light having at least one predeterminable wavelength;
• and
• a single detection unit adapted to detect the fluorescence light from the plurality of cavities of each sample carrier;
• and
• a coupling module including a coupling receptacle configured to receive at least one light guide configured to guide the excitation light from the fluorescence unit to each measuring head and to guide the fluorescence light from each measuring head to the fluorescence unit and to the single detection unit therein.