Analyte quantitation using Raman spectroscopy
AssigneeOndaVia, Inc.
InventorsMark Charles Peterman, Merwan Benhabib
Life of this patent
Estimating the term…
Abstract
An analyte in water is quantified by mixing an internal standard with a water sample. The sample is analyzed with a Raman spectrometer. The analysis includes mapping the signal from the Raman spectrometer to the quantity of said analyte. The mapping can include using a calibration curve, for example a multivariate calibration curve, that was previously prepared with the internal standard. In other examples, a sample is measured after addition of a known amount of the analyte.
Claims20
A method to measure an analyte in water comprising the steps of:
- providing a sample containing a quantity of said analyte;
- mixing an internal standard with the sample, wherein the internal standard comprises an isotope;
- analyzing the sample with a Raman spectrometer to produce a measured spectrum of the sample;
- and,
- determining a concentration of the analyte using the measured spectrum and a calibration curve wherein the calibration curve is one or more of a) a multivariate calibration curve and b) a calibration curve prepared with samples containing the analyte and the internal standard;
- wherein determining a concentration of the analyte using the measured spectrum comprises:
- collecting multiple spectra of the sample at multiple excitation wavelengths,
- obtaining variant and invariant spectra, and
- determining concentration of the analyte using the variant spectrum.
A method to measure an analyte in water comprising the steps of:
- providing an original sample containing a quantity of said analyte;
- mixing one or more known amounts of the analyte with the sample to produce at least one additional sample;
- analyzing the original sample and the at least one additional sample with a Raman spectrometer to produce measured spectra of the samples;
- and,
- determining a concentration of the analyte using the measured spectra
- wherein determining a concentration of the analyte using the measured spectra comprises:
- collecting multiple spectra of the sample at multiple excitation wavelengths,
- obtaining variant and invariant spectra, and
- determining concentration of the analyte using the variant spectrum.
A method of measuring the concentration of MEA-triazine in a sample comprising adding a Raman-active compound containing a phenyl group to the sample and producing a Raman spectra of the sample.
Dependencies are inferred from the claim text.