Back to results
US 11,993,818 B2Granted
Methods for characterizing cell-free nucleic acid fragments
AssigneeAqtual, Inc.
InventorDiana Abdueva
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods and systems for various uses of cell-free nucleic acid (cfNA). Functional typing of cfNA fragmentation patterns may be utilized in the non-invasive detection, diagnosis, and monitoring of disease. One embodiment may determine a stage of cancer in a subject, the progression of cancer in a subject, or the responsiveness to treatment of a cancer in a subject. Another embodiment disclosed herein may include sequencing-free diagnostic methods.
Claims19
A method of characterizing cell-free DNA (cfDNA) fragments comprising a sequence of a genomic region, comprising:
- (a) contacting said cfDNA fragments with:
- (i) a first oligonucleotide bait that hybridizes to a first portion of said genomic region, and (ii) a second oligonucleotide bait that hybridizes to a second portion of said genomic region;
- and
- (b) detecting:
- (i) an amount of said cfDNA fragments that comprise said first portion of said genomic region;
- and (ii) an amount of said cfDNA fragments that comprise said first portion of said genomic region and said second portion of said genomic region using said first oligonucleotide bait and said second oligonucleotide bait,
- wherein said first nucleotide bait is configured to hybridize to a cfDNA of less than 200 nucleotides in length and a cfDNA of greater than 200 nucleotides in length,
- wherein said second oligonucleotide bait is configured to hybridize to said cfDNA of greater than 200 nucleotides in length preferentially over said cfDNA of less than 200 nucleotides in length, and
- wherein said genomic region comprises at least part of a promotor, a transcriptional start site, a DNAse I-hypersensitive site, a Pol II pausing site, a first exon, or an intron to exon boundary.
6 more elements
Dependencies are inferred from the claim text.