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US 11,993,805 B2Granted
Methods, compositions, and kits for preparing nucleic acid libraries
AssigneeGenefirst Ltd.
InventorsGuoliang Fu, Thomas Dunwell
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Abstract
This invention relates to methods, compositions and kits for extending a polynucleotide and for preparing sequencing library of polynucleotides involving generating modified target polynucleotide on an adaptor template oligonucleotide and tagging one or two strands of a target sequence. The sequencing library is suitable for massive parallel sequencing and comprises a plurality of double-stranded nucleic acid molecules.
Claims9
An adaptor template oligonucleotide (ATO) for extending polynucleotides comprising:
- (a) a 3′ random sequence of 3 to 36 ‘N’ bases;
- (b) a 3′ end with a blocker, which renders ATO non-extendible;
- (c) a universal sequence, 5′ to the random sequence;
- (d) modified nucleotides or linkages which render the ATO resistant to 3′ exonuclease cleavage;
- and
- (e) a uracil nucleotide which renders the ATO degradable.
3 more elements
Dependencies are inferred from the claim text.