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US 11,993,794 B2Granted
Methods to create chemically-induced dimerizing protein systems for regulation of cellular events
AssigneeFred Hutchinson Cancer Center
InventorRupesh Amin
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Abstract
Methods to create chemically-induced dimerizing (CID) protein systems and uses thereof are described. The methods utilize antibody binding domain dimerizing proteins. The created systems can be used to regulate cellular events such as gene expression, receptor signaling and cell death to effectuate a variety of clinically relevant treatment outcomes.
Claims18
A method of regulating cellular events within a cell, comprising:
- administering a cell-permeable small molecule to the cell, wherein the cell has internalized genetic constructs encoding:
- a first fusion protein comprising an antibody binding domain (AB1) and a first effector molecule, wherein AB1 binds the small molecule to form an AB1/small molecule complex, and
- a second fusion protein comprising a metatype antibody binding domain (AB2) and a second effector molecule, wherein AB2 binds a portion of AB1 and a portion of the small molecule, but only when AB1 is bound to the small molecule to form an AB1/small molecule complex;
- wherein upon the administering of the cell-permeable small molecule, the first fusion protein and the second fusion protein dimerize to regulate cellular events within the cell.
1 more element
Dependencies are inferred from the claim text.