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US 11,993,767 B2Granted
Method for producing 3D, biocompatible polymer scaffold with a cell-filled cavity
AssigneeCELLBRICKS GmbH
InventorsLutz Kloke, Alexander Thomas, Tobias Lam
Life of this patent
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Abstract
A 3D scaffold of a biocompatible polymer and colonized with biological cells is provided. The biological cells can be cultured to form a 3D cell culture construct that closely approximates a physiological architecture. A method for producing the 3D scaffold colonized with biological cells is also provided.
Claims17
A method including:
- (a) constructing a 3D scaffold comprising a biocompatible polymer using a lithographic 3D printing method, the 3D scaffold having an at least partially covered cavity and wherein the biocompatible polymer is obtained by rendering a biopolymer as a starting substance photopolymerizable or photocrosslinkable by introducing a photoreactive group, the biopolymer being selected from a group consisting of proteins, DNA, RNA, carbohydrates, carbohydrate derivatives, collagens, fibrins, alginates, gelatins, and hyaluronic acids;
- and
- (b) filling the at least partially covered cavity with a suspension containing biological cells to colonize the 3D scaffold.
A 3D scaffold comprising:
- (a) multiple layers of a biocompatible polymer, the multiple layers of the biocompatible polymer formed by a lithographic 3D printing method and defining an at least partially covered cavity and wherein the biocompatible polymer is obtained by rendering a biopolymer as a starting substance photopolymerizable or photocrosslinkable by introducing a photoreactive group, the biopolymer being selected from a group consisting of proteins, DNA, RNA, carbohydrates, carbohydrate derivatives, collagens, fibrins, alginates, gelatins, and hyaluronic acids;
- and
- (b) a suspension containing biological cells filling the at least partially covered cavity to colonize the 3D scaffold.
Dependencies are inferred from the claim text.