Functionalized well plate, methods of preparation and use thereof
AssigneeBruker Cellular Analysis, Inc.
InventorsPeter J. Beemiller, Alexander J. Mastroianni, Randall D. Lowe, Jr., Yelena Bronevetsky
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Abstract
In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces of wells within a well plate to activate lymphocytes, including but not limited to T lymphocytes, in a controllable and reproducible manner.
Claims27
A well plate comprising a surface for presenting an antigen, wherein the surface has a first region which is an activating moiety-presenting covalently functionalized region comprising a plurality of specifically bound primary activating molecular ligands and a plurality of specifically bound co-activating molecular ligands,
- wherein the first region has an area of 2 mm2 to 35 mm2.
A kit for preparing a well plate comprising a surface for presenting an antigen, wherein the surface has an activating moiety-presenting covalently functionalized region, comprising:
- a well plate comprising a surface comprising a reactive moiety-presenting covalently functionalized region, wherein the reactive moiety is an azido moiety, an alkynyl moiety, a biotin moiety, or a streptavidin moiety;
- and
- an activating reagent comprising a primary activating reagent and a co-activating reagent,
- wherein the reactive moiety-presenting covalently functionalized region has an area of 2 mm2 to 35 mm2.
Dependencies are inferred from the claim text.