Immobilized microbial agent for in situ restoration of contaminated sediments, preparation method and application thereof

An immobilized microbial agent for in situ restoration of contaminated sediments, composed of a Hangjin clay 2 # particular filler-loaded with conductive microorganisms, obtained by the following method:

• pretreating the Hangjin clay 2 # to obtain the particulate filler, the Hangiin clay 2# comprising quartz, feldspar, calcite and amorphous iron oxide;
• culturing and amplifying the conductive microorganisms to produce a bacterial liquid to be inoculated;
• adding the particulate filler into the bacteria liquid;
• mixing the particulate filler and the bacterial liquid under anaerobic conditions to provide a mixture;
• and
• removing a supernatant after letting the mixture stand, allowing solid and liquid phases to separate, thereby obtaining the immobilized microbial agents,
• wherein the conductive microorganisms include Geobacter sulfurreducens, Geobacter metallireducens and Shewanella oneidensis.