Adsorption medium, method for production thereof, and use thereof for purification of biomolecules
AssigneeSartorius Stedim Biotech GmbH
InventorsLouis Villain, Florian Taft, Jan Schwellenbach
Life of this patent
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Abstract
The present invention relates to an adsorption medium including polymeric space elements which have been bonded to the surface of the chromatography matrix, and polymer chains containing chromatographically active centers, wherein the polymer chains have been bonded to the polymeric spacer elements, as well as to a method for the production thereof, and to the use of the adsorption medium for the purification of biomolecules.
Claims21
An adsorption medium comprising
- a chromatography matrix having a surface, the chromatography matrix selected from the group consisting of natural or synthetic fibers, polymer membranes, nonwovens and wovens;
- polymeric spacer elements having a molecular weight per polymeric spacer element ranging from 5000 to 2,000,000 g/mol, wherein the polymeric spacer elements are selected from the group consisting of polyamines and polyvinyl alcohols, and wherein the polymeric spacer elements are directly chemically or physically bonded to the surface of the chromatography matrix;
- and
- poly(meth)acrylate polymer chains containing chromatographically active centers, wherein the chromatographically active centers are selected from the group consisting of anionic and cationic groups and wherein the poly(meth)acrylate polymer chains are chemically bonded to the polymeric spacer elements.
A method for producing an adsorption medium, comprising the steps:
- (a) providing a chromatography matrix having a surface, the chromatography matrix selected from the group consisting of natural or synthetic fibers, polymer membranes, nonwovens and wovens;
- (b) immobilizing polymeric spacer elements directly chemically or physically on the surface of the chromatography matrix, the polymeric spacer elements having a molecular weight per polymeric spacer element ranging from 5000 to 2,000,000 g/mol, wherein the polymeric spacer elements are selected from the group consisting of polyamines and polyvinyl alcohols;
- and
- (c) immobilizing poly(meth)acrylate polymer chains containing chromatographically active centers chemically on the polymeric spacer elements to produce the adsorption medium, wherein the chromatographically active centers are selected from the group consisting of anionic and cationic groups.
A method of purifying biomolecules using an adsorption medium comprising the steps of:
- providing an adsorption medium including a chromatography matrix having a surface, the chromatography matrix selected from the group consisting of natural or synthetic fibers, polymer membranes, nonwovens and wovens;
- polymeric spacer elements directly chemically or physically bound to the chromatography matrix, the polymeric spacer elements having a molecular weight per polymeric spacer element ranging from 5000 to 2,000,000 g/mol and wherein the polymeric spacer elements are selected from the group consisting of polyamines and polyvinyl alcohols;
- and poly(meth)acrylate polymer chains containing chromatographically active centers, wherein the chromatographically active centers are selected from the group consisting of anionic and cationic groups and wherein the poly(meth)acrylate polymer chains are chemically bound to the polymeric spacer elements;
- and
- passing a solution containing the biomolecules across the adsorption medium to obtain purified biomolecules.
Dependencies are inferred from the claim text.