Chimeric influenza vaccines
AssigneeAcademia Sinica
InventorsChi-Huey Wong, Hsin-Yu Liao, Shih-Chi Wang, Yi-An Ko, Kuo-I Lin, Che Ma, Ting-Jen Cheng
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Abstract
The present disclosure relates to a chimeric influenza virus hemagglutinin (HA) polypeptide, comprising one or more stem domain sequence, each having at least 60% homology with a stem domain consensus sequence of H1 subtype HA (H1 HA) and/or H5 subtype HA (H5 HA), fused with one or more globular head domain sequence, each having at least 60% homology with a globular head domain consensus sequence of H1 subtype HA (H1 HA) or H5 subtype HA (H5 HA).
Claims21
An immunogenic composition comprising a monoglycosylated chimeric influenza virus hemagglutinin (HA) polypeptide and an adjuvant;
- wherein the monoglycosylated chimeric influenza virus HA polypeptide comprising an amino acid sequence of SEQ ID NO:
- 4;
- wherein the monoglycosylated chimeric influenza virus HA polypeptide has only N-Acetylglucosamine (GlcNAc) at N28, N40, N182, N292, and N497;
- and
- wherein the adjuvant is selected from the group consisting of Al(OH)3, AlPO4, an analog of α-galactosylceramide (α-GalCer), squalene and QS21.
A method for preparing a chimeric influenza virus hemagglutinin (HA) polypeptide Swap H1/5, having one or more stem domain sequence fused with one or more globular head domain sequence, comprising:
- composing H1 as HA1 (amino acids 1-327 of SEQ ID NO:
- 8) And H5 as HA2 (amino acids 328503 of SEQ ID NO:
- 8) giving H1 as globular head and [H1+H5 (HA2)] as stem.
A method for preparing a chimeric influenza virus hemagglutinin (HA) polypeptide Swap H5/1, having one or more stem domain sequence fused with one or more globular head domain sequence, comprising:
- composing H5 as HA1 (amino acid 1-330 of SEQ ID NO:
- 12) And H1 as HA2 (amino acid 331-506 of SEQ ID NO:
- 12) giving H5 as globular head and [H5+H1 (HA2)] as stem.
A method for preparing a chimeric influenza virus hemagglutinin (HA) polypeptide chimeric H5/1, having one or more stem domain sequence fused with one or more globular head domain sequence, comprising:
- (a) constructing the globular head domain with the amino acid sequence between residues C42 and C274 of SEQ ID NO:
- 4 (H3 numbering);
- and
- (b) constructing the stem domain with portions of HA1 and HA2 subunits (amino acids 1˜41 of SEQ ID NO:
- 4 and 275-511 of SEQ ID NO:
- 4).
Dependencies are inferred from the claim text.