Cell-mediated transient delivery of immune-enhancing molecules into the tumor microenvironment

A recombinant natural killer (NK)-92 cell transfected with a plasmid expression vector and gamma (γ)-irradiated,

• wherein the plasmid expression vector comprises a nucleic acid that encodes i) a homing receptor;
• ii) an antigen binding protein (ABP) or a chimeric antigen receptor (CAR) that specifically binds a target antigen;
• iii) an Fc Receptor;
• and iv) a secreted immune modulator, wherein the secreted immune modulator is a TGFβ inhibitor,
• wherein the homing receptor is selected from the group consisting of a G protein-coupled receptor, a chemokine receptor, a cytokine receptor, a cell adhesion molecule, a selectin, and an integrin,
• wherein the TGFβ inhibitor has the amino acid sequence of SEQ ID NO:64 or SEQ ID NO:66 and
• wherein the nucleic acid is operably linked to a promoter.