Entomopathogenic nematodes (EPN) species as a biological delivery system
AssigneeThe State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center)
InventorsItamar Glazer, Liora Salame, Dana Ment, Neta Morag
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Abstract
Disclosed are beneficial complexes comprising entomopathogenic nematodes (EPNs), binders, and external cargos, methods for preparing the same and systems comprising the same.
Claims19
A complex, comprising:
- (a) an entomopathogenic nematode (EPN),
- (b) a cargo composition attached to the external surface of the EPN, and
- (c) a binder composition that binds the external cargo composition to the external surface of the EPN
- wherein the cargo composition comprises a spore of a fungus, and wherein the binder composition comprises a lectin.
A method for preparing a complex, wherein the complex comprises an entomopathogenic nematode (EPN), a cargo composition attached to the external surface of the EPN, and a binder composition that binds the external cargo composition to the external surface of the EPN, the method comprising the steps of
- (a) incubating the EPN with the binder composition to prepare an EPN-binder complex,
- (b) incubating the cargo composition with the binder composition to prepare a cargo-binder complex, and
- (c) incubating the EPN-binder complex with the cargo-binder complex, thereby preparing the EPN-binder-cargo complex,
- wherein the cargo composition comprises a spore of a fungus, and wherein the binder composition comprises a lectin.
A method of killing or sustainably inhibiting a pest, comprising the step of contacting the pest with a complex comprising an entomopathogenic nematode (EPN), a cargo composition attached to the external surface of the EPN, and a binder composition that binds the external cargo composition to the external surface of the EPN, wherein the cargo composition increases the pathogenicity of the EPN towards the pest, wherein the cargo composition comprises a spore of a fungus, and wherein the binder composition comprises a lectin.
Dependencies are inferred from the claim text.