Methods of heat inactivation of adenovirus
AssigneeUltragenyx Pharmaceutical Inc.
InventorsChristopher J. Morrison, James D. Maratt
Life of this patent
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Abstract
The present disclosure generally relates to methods of protecting the genomic integrity and/or biological activity of AAV viral particles in a sample containing both AAV particles and helper virus particles during heat inactivation. The methods include heating, to a temperature greater than or equal to 45′C, a sample containing helper virus particles, AAV particles, and a buffer. The buffer includes a concentration of 10 mM or greater kosmotropic salts and/or a concentration of 10 mM or greater of divalent or trivalent cations.
Claims20
A method of inactivating helper virus in a sample containing helper virus, adeno-associated virus particles, and a buffer comprising a concentration of 25 mM to 500 mM of divalent or trivalent cations, or a concentration of 0.5 M to 0.6 M of kosmotropic salts, wherein the method comprises:
- heating the sample to a temperature of 45° C. to 55° C.,
- and maintaining the sample at the temperature for a time period of 1 minute to 6 hours, thereby inactivating helper virus.
Dependencies are inferred from the claim text.