Glycan analysis method
AssigneeShimadzu Corporation
InventorTakashi Nishikaze
Life of this patent
Estimating the term…
Abstract
Glycans having a branched structure are labeled with a labeling agent, such as 2-aminobenzoic acid, having one site that is easily negatively charged. At this time, reduction which is usually performed in labeling by reduction amination is not performed. A sample for mass spectrometry containing the labeled form of glycans thus obtained is prepared, and is subjected to MS/MS analysis in negative ion mode. In the MS/MS spectra obtained by the MS/MS analysis, peaks of E ions, D ions and the like which reflect the branched structure clearly appear. As a result, structural analysis of an entirety of the glycan including the branched structure can be easily performed.
Claims9
A glycan analysis method for analyzing a structure of glycan using mass spectrometry, the glycan analysis method comprising:
- a) a sample preparation step of preparing a sample by labeling glycan to be analyzed without reduction, using an acidic labeling agent having at least one site stably presenting a negative charge in a molecule with the acid labeling agent bound to the glycan;
- and
- b) an analysis execution step of subjecting the sample to MS/MS analysis in negative ion mode, where a deprotonated ion of the glycan labeled in step a is used as a precursor ion;
- c) a detecting step of detecting a product ion of D ion or E ion which reflects a branched structure of the glycan from a result of the MS/MS analysis;
- and
- d) an estimating step of estimating a structure of the glycan based on step c,
- wherein the site that stably presents a negative charge in the acidic labeling agent is a carboxy group.
Dependencies are inferred from the claim text.